Application

Anti-Peripherin, clone 1D4 ZooMAb , Cat. No. ZRB1174, is a highly specific rabbit recombinant monoclonal antibody that targets Peripherin and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Peripherin in mouse spinal cord tissue lysate.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Peripherin in human esophagus and rat spinal cord tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Peripherin in PC12 and SH-SY5Y cells.Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Peripherin.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

30 µg/mL after reconstitution at 25 µL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 1D4 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Peripherin. It targets an epitope within 17 amino acids from the C-terminal region.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Peripherin (UniProt: P41219; also known as Neurofilament 4) is encoded by the PRPH (also known as NEF4, PRPH1) gene (Gene ID: 5630) in human. Peripherin is a member of the intermediate filament family. It is classified as a class-III neuronal intermediate filament protein that is expressed mainly in neurons of the peripheral nervous system. It is also found in neurons of the central nervous system that have projections toward peripheral structures, such as spinal motor neurons. Its expression is also reported in certain neuroendocrine tumors. Peripherin is shown to coexist with the neurofilament triplet proteins (neurofilament light, medium, and heavy chain). PRPH gene expression is reported to be the highest in late embryonic and early postnatal brain and declines somewhat before expression of triplet protein sharply rises. A possible peripherin involvement in amyotrophic lateral sclerosis (ALS) pathogenesis has been suggested based on studies with transgenic mice that display over-expression and with a toxic splicing variant of the gene. Two isoforms of peripherin have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Leung, CL., et al., (2004). Brain Pathol. 14(3); 290-296; Yuan, A., et al. (2012). J. Neurosci. 32(25); 8501-8508).